Controlled autolysis facilitates the polyhydroxyalkanoate recovery in Pseudomonas putida KT2440

Controlled autolysis facilitates the polyhydroxyalkanoate recovery in Pseudomonas putida KT2440

The development of efficient recovery processes is essential to reduce the cost of polyhydroxyalkanoates (PHAs) production. In this work, a programmed self-disruptive Pseudomonas putida BXHL strain, derived from the prototype medium-chain-length PHA producer bacterium P. putida KT2440, was constructed as a proof of concept for exploring the possibility to control and facilitate the release of P...

Valerolactone from Levulinate in Pseudomonas putida KT2440

Amino acid racemization in Pseudomonas putida KT2440.

D-Amino acids have been shown to play an increasingly diverse role in bacterial physiology, yet much remains to be learned about their synthesis and catabolism. Here we used the model soil- and rhizosphere-dwelling organism Pseudomonas putida KT2440 to elaborate on the genomics and enzymology of d-amino acid metabolism. P. putida KT2440 catabolized the d-stereoisomers of lysine, phenylalanine, ...

Genome-Scale Reconstruction and Analysis of the Pseudomonas putida KT2440 Metabolic Network Facilitates Applications in Biotechnology

A cornerstone of biotechnology is the use of microorganisms for the efficient production of chemicals and the elimination of harmful waste. Pseudomonas putida is an archetype of such microbes due to its metabolic versatility, stress resistance, amenability to genetic modifications, and vast potential for environmental and industrial applications. To address both the elucidation of the metabolic...

Monitoring differences in gene expression levels and polyhydroxyalkanoate (PHA) production in Pseudomonas putida KT2440 grown on different carbon sources.

Pseudomonas putida has a variety of potential uses in bioremediation and biosynthesis of biodegradable plastics. P. putida is able to utilize a wide range of carbon sources. In this study, P. putida KT2440 was grown on glucose, glycerol, citrate, or fatty acid (lauric acid) as the sole carbon source. Differences in expression levels of genes involved in the Entner-Doudoroff pathway, glycerol me...